top of page

Methodologies

Instrumental Analytics
​

 

  • HPLC (refraction index, fluorescence and diode array detection)
  • Capillary electrophoresis (diode array detection)
  • Dissolution testing

In-capillary reactions

​

  • Enzymatic reaction kinetics using capillary electrophoresis

Caco-2 model

PAMPA model

In vitro permeability and transport models

​

  • PAMPA model
  • Bidirectional Caco-2 model

Cytotoxicity

​

  • MTS/MTT

Protein Characterization

​

  • SDS-PAGE
  • Western immunoblot

Whole-cell assays development

​

  • Spectrophotometric assays
  • Fluorimetric assays
  • In-gel activity assays

PHARMACOKINETICS AND BIOPHARMACEUTICAL ANALYSIS

bottom of page